He was selected by the Miami Dolphins in the second round of the 2001 NFL draft, and also played for the San Diego Chargers and Kansas City Chiefs.

After his playing career, Chambers served in 2021 as the first-ever University of Fort Lauderdale head football coach.

He was a high school teammate of wide receiver Lee Evans, who also played for the Wisconsin Badgers.

Chambers topped the football team in receiving his sophomore season when he totaled 28 catches for 563 yards and seven touchdowns, including an 80-yard grab against Michigan.

He missed two games as a junior with a broken finger, but still led the team in receiving with 41 catches for 578 yards and four scores, and was an honorable mention All-Big Ten selection.

Despite playing in only nine games as a senior because of a stress fracture in his right foot, Chambers put together the most productive season of his career with 52 receptions for 813 yards and five touchdowns.

Chambers missed the team's first-round playoff game on January 13, 2002, against the Baltimore Ravens due to a sprained ankle suffered the last week of the regular season.

He missed the team's October 20 contest against the Buffalo Bills while recovering from a concussion suffered on a hit by then-Denver Broncos safety Kenoy Kennedy.

Chambers' yardage total was the most by a Dolphin since Martin had 1,037 yards in 1999, and his 11 touchdowns were the most since Mark Clayton hauled in 12 receiving scores in 1991.

His average per reception of 15.0 tied for the ninth-highest figure among the top 50 receiving yardage leaders in the AFC.

It marked the fourth straight year in which Chambers led the team in receiving yardage, which made him just the third Dolphin ever to achieve this feat, joining Paul Warfield (1970–1973) and Nat Moore (1974–1977).

He also rushed for 76 yards on nine attempts during the course of the season, including a season-long 24-yard run against the Arizona Cardinals on November 7.

Chambers accounted for four 100-yard receiving games on the year, tied for the sixth-highest single-season figure in club history and the most by a Dolphin since Tony Martin had five in 1999.

His reception total tied for sixth in the AFC and 13th in the NFL, while his yardage figure was third in the conference and 12th in the league.

His eleven touchdown catches tied for the second-highest figure in the conference and third in the NFL, trailing only Indianapolis' Marvin Harrison (12) and Carolina's Steve Smith (12).

In the game, which the Dolphins trailed by 20 points entering the fourth quarter, Chambers caught 15 passes for 238 yards and a touchdown.

Key receptions included a 57-yard grab on the final drive with under two minutes left, as well as the game-winning touchdown on a pass from Sage Rosenfels with only six seconds remaining.

The Dolphins' offense struggled as a whole, and he never seemed to be a favorite target for quarterback Joey Harrington, and finished third on the team in both receptions and receiving yards.

He was held without a catch on December 17 at the Buffalo Bills, breaking his streak of making at least one reception in 60 consecutive games.

Signed through 2009, Chambers was reunited with Chargers coach Norv Turner, who was Miami's offensive coordinator in 2002–03.

Chambers' was inactive in weeks 6 and 7 due to an ankle injury sustained during a game against the Miami Dolphins.