[8][9] Born in Turin, to a father from Piedmont and a mother from Sicily, Marchisio is the youngest of three children and was raised in the comuni of Chieri and Andezeno, where his parents run a family business and continue to reside.

After being included in the preseason friendlies, Marchisio was promoted to the first team permanently following Juventus' relegation to Serie B due to the Calciopoli scandal and given the number 15 shirt.

On 19 August 2006, he made his first team debut in the Coppa Italia third round, coming on as a late substitute for Matteo Paro in a 3–0 win over Martina.

[13] He capped a fine season with an assist for Del Piero's match opener in the 5–1 thrashing of Arezzo,[22] a result which secured immediate promotion to Serie A mathematically.

On 25 July 2007, Marchisio was loaned out to Empoli for the 2007–08 season with good friend and fellow youth product Sebastian Giovinco to gain first-team experience.

In November, former Milan and Fiorentina striker Stefano Borgonovo wrote about him on La Gazzetta dello Sport: "Marchisio has everything needed to play at the highest level...[He] has personality, reminds me of the great Marco Tardelli.

[15][29] His season was interrupted by a series of minor injuries in April, which resulted in him being sidelined for almost a month, and he returned to the starting eleven for the penultimate matchday away at Siena, marking it with a goal and an assist for captain Alessandro Del Piero's second goal of the match in a 0–3 win[30] and then setting up Vincenzo Iaquinta's opener in a 2–0 home win against Lazio, a result that ensured a second-place finish for the Bianconieri.

[34] After surgery, he was sidelined for one month and a half and made his comeback as a last-minute substitute in the Champions League group stage tie away at Bordeaux on 25 November.

On 5 December 2009, he scored the winner in a 2–1 home win over Internazionale in the Derby d'Italia, where he showed incredible composure to drag the ball with both feet before dinking it over the stranded Júlio César.

[35] Three days later, he set up David Trezeguet to score the opening goal of the game in the decisive Champions League 1–4 home loss against Bayern Munich.

[41] Marchisio started the 2011–12 season well by scoring Juve's final goal in the opening match of the campaign at the club's new stadium, a 4–1 home win over Parma on 11 September 2011.

[42][43] Under former Juventus legend Antonio Conte, he was played alongside new signings Arturo Vidal and Andrea Pirlo to great effect and were dubbed the "M-V-P" midfield by the press and fans.

In November, he scored his first-ever goal in a UEFA club competition in the Champions League group stage home fixture against Nordsjælland.

[62][63] In the absence of Andrea Pirlo due to injury, Marchisio occasionally deputised as designated corner and indirect free kick taker.

[65] In the Champions League Final loss to Barcelona, he started the counterattack with a backheel pass that led to Álvaro Morata's equalizer, but the eventual winners scored two more goals to win 3–1.

[69][70] Marchisio was ruled out for a month after sustaining an injury to an adductor muscle in his right leg in his first league appearance of the season on 12 September 2015, as Juventus drew 1–1 at home to Chievo.

[74] Two weeks later on 17 April, Marchisio suffered an anterior cruciate ligament injury during Juventus' 4–0 home victory over Palermo after an awkward fall with a challenge on Franco Vázquez, which ruled him out for the rest of the season as well as the upcoming UEFA Euro 2016 tournament.

[101] On 12 August 2009, Marchisio made his senior debut, starting in the friendly against Switzerland that ended goalless[102][103] and was praised by Marcello Lippi during the post-match interview.

[104] Lippi later revealed that he had intended to call Marchisio up earlier but agreed to leave the youngster to Casiraghi's U-21 squad until the end of the European Championships.

Marchisio played his first competitive match for the Azzurri in the World Cup qualifier against Bulgaria in his home stadium and became a first-choice pick for Lippi's squad.

[105] He was forced to withdraw, however, from the remaining qualifying matches against the Republic of Ireland and Cyprus after being diagnosed with a knee meniscus injury that required surgery.

[119][120][121] The referee's failure to send Luis Suárez off for biting Giorgio Chiellini, and the fact that Uruguay went on to win 1–0 after scoring from a corner immediately following the later incident, further added to the controversy.

[134] He has also occupied a deeper playmaking role for his team, in particular after Pirlo's departure in 2015, in which he has been praised for his ability to spread the ball wide, or dictate play in midfield with his passing.

During the 2011–12 season at club level, he was deployed effectively alongside Pirlo and Chilean international Arturo Vidal in Juventus's three-man midfield in their 3–5–2 formation under Conte.

A large part of Juventus's success in Italy that season was due to the fact that Marchisio and Vidal were able to interchange quickly between defensive and attacking positions and compensate for Pirlo's defensive weaknesses by leaving playmaking duties to the latter; this formation allowed all three midfielders to contribute with 19 league goals, almost a third of all goals scored by the team that season, as they went on to win the league.

[156][157] Since 2014, at club level, with Massimiliano Allegri's switch in tactics, as well as Marchisio's injury struggles, and Pirlo's subsequent departure in 2015, Marchisio has been played in a much deeper, creative role, usually in front of the back-line, where he effectively provides the link between defence and offence by intercepting balls and disrupting the opposition's play where necessary, while also looking to orchestrate attacking moves with his passing after winning back possession.

Instead, he’s consistent, a sort of Italian Denis Irwin: lots of matches where the good work goes unnoticed, plenty of seven/eight out of 10 showings.One of Marchisio's key strengths is his ability to switch from defence to offence seamlessly after winning back the ball, while remaining tactically disciplined in either role;[161][162][163] his athleticism, tackling, vision, range of passing, and good reading of the game enables him to break down the opposition's play,[8][144][164][165] and quickly transition the ball forward to create chances for his more attack-minded teammates.

[128][144][166][167][168][169][170] These attributes, as well as his ability to pass and shoot with both feet, despite being naturally right-footed, make him a threat in the opponent's half, especially in or outside the penalty area; if given the chance, he has been known to attempt a shot on goal, either from distance, or by finishing off a teammate's pass after making late runs from behind into the area,[9][128][134][157][171][172][173][174] – as exhibited by his goals against Cagliari in January 2014,[175] Luxembourg in a June 2014 friendly,[176] and England at the 2014 FIFA World Cup.

[177] Due to his composed yet hard-working and tenacious style of play, Marchisio has garnered comparisons to Juventus legend and 1982 World Cup winner Marco Tardelli by the Italian press,[13][166][178][179] who was arguably one of Italy's greatest "two-way" midfielders.

[193] The idea was born out of his own experience and the realisation about professional athletes' lack of knowledge or guidance on their legal rights, leaving them vulnerable to unscrupulous agents or business representatives.