The Colocalization Benchmark Source (CBS) is a free collection of downloadable images to test and validate the degree of colocalization of markers in any fluorescence microscopy studies.

Colocalization is a visual phenomenon when two molecules of interest are associated with the same structures in the cells and potentially share common functional characteristics.

[1][2][3] CBS provides researchers with reference tools to verify the results of quantitative colocalization measurements.

[4] It serves as a specialised bioimage informatics database of computer-simulated images with exactly known (pre-defined) values of colocalization.

The template for submitting information about custom images can be downloaded from CBS site.