[3] Colombia fielded a squad of two artistic swimmers to compete in the women's duet event, by finishing ninth and securing the last of the eight available spots at the 2021 FINA Olympic Qualification Tournament in Barcelona, Spain.

[9][10] Colombia entered three divers into the Olympic competition by virtue of a top twelve finish in the men's springboard at the 2019 FINA World Championships and by winning the gold medal in the same event at the 2019 Pan American Games in Lima, Peru.

Colombia entered one equestrian rider into the Olympic competition by finishing among the top ten and securing the second of four available slots in the individual jumping at the 2019 Pan American Games in Lima, Peru.

Set to compete at her third consecutive Games, Saskia Loretta van Erven Garcia claimed a spot in the women's foil as the top-ranked fencer vying for qualification from the Americas in the FIE Adjusted Official Rankings.

Set to compete at her fourth straight Games, London 2012 bronze medalist Yuri Alvear accepted a continental berth from the Americas as the nation's top-ranked judoka outside of direct qualifying position in the IJF World Ranking List of June 28, 2021.

[20] Colombia granted an invitation from ISSF to send Bernardo Tobar Prado in the men's rapid fire pistol to the Olympics, as long as the minimum qualifying score (MQS) was fulfilled by June 6, 2021.

Juan Sebastián Cabal and Robert Farah qualified directly for the men's doubles by virtue of their combined top 10 placement in the ATP World Rankings of 14 June 2021.