[3] On December 6, 2021, the Columbus Crew announced the formation of a reserve team in MLS Next Pro that would begin play in the 2022 season and that they would play at Historic Crew Stadium (the parent club's former home venue) in Columbus, Ohio.

[6] On January 28, 2022, the Crew announced that Laurent Courtois would serve as the team's first head coach.

[7] The club played their first match on March 26, 2022, which ended in a 0–2 loss to Inter Miami CF II at DRV PNK Stadium.

[9] Crew 2 won the first ever MLS Next Pro Cup title, defeating St. Louis City SC 2 4-1 on October 8, 2022.

[11] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.