In molecular biology, the condensation domain is a protein domain found in many multi-domain enzymes which synthesise peptide antibiotics.

This domain catalyses a condensation reaction to form peptide bonds in non-ribosomal peptide biosynthesis.

It is usually found to the carboxy side of a phosphopantetheine binding domain (pp-binding).

It has been shown that mutations in the HHXXXDG sequence motif in this domain abolish activity suggesting this is part of the active site.

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