Route 164 is a minor state highway in southeastern Connecticut, United States, running from Route 2 in Preston through the village of Preston City to Route 138 in Griswold just south of the borough of Jewett City.

Route 164 begins as Preston Plains Road at an intersection with Route 2 in the southeastern portion of the town of Preston.

Route 164 continues for another 0.3 miles (0.48 km) beyond the interchange to end at Route 138 just south of the borough of Jewett City.

[1] A section of Route 164 in Preston from the center of Preston City to the Griswold town line is designated a scenic road.

[1] The direct route from the village of Preston City to the borough of Jewett City was established as Route 164 in the 1932 state highway renumbering.