Cot analysis

[2] The procedure involves heating a sample of genomic DNA until it denatures into the single stranded-form, and then slowly cooling it, so the strands can pair back together.

[2] This allows DNA sequencing to concentrate on the parts of the genome that are most informative and interesting, which will speed up the discovery of new genes and make the process more efficient.

[3][4] It was first developed and utilized by Roy Britten and his colleagues at the Carnegie Institution of Washington in the 1960s.

[5][6] Of particular note, it was through C0t analysis that the redundant (repetitive) nature of eukaryotic genomes was first discovered.

In fact, their experiments demonstrated that the majority of eukaryotic genomic DNA is composed of repetitive, non-coding elements.