She was married on 9 September 1591 in Neuburg to Duke Frederick William I of Saxe-Weimar (1562–1602).

On the occasion of the marriage, a medal was minted in gold, representing the couple, one on each side with a bust.

After she was widowed in 1602, Anna Maria sank into deep sadness and from 1612, she lived separate from her children on her Wittum, the District and City of Dornburg.

Out of gratitude for this assistance, the Duchess donated a chalice to the local church.

Anna Maria died in 1643 and was buried in the brick royal crypt in the Brethren Church in Altenburg.