The song was a success on its initial release and was certified Gold by the RIAA on August 18, 1971, and Platinum on April 10, 2017.

"[8] To Danoff, the lyric "[t]he radio reminds me of my home far away" in the bridge is quintessentially West Virginian, an allusion to when he listened to the program Saturday Night Jamboree, broadcast from Wheeling, West Virginia, on WWVA at his home in Springfield, Massachusetts, during his childhood in the 1950s.

[9] Of the commune members, Danoff remarked, "They brought their dogs and were a very colorful group of folks, but that is how West Virginia began creeping into the song."

According to Len Jaffe, a Washington, D.C.-based singer-songwriter who attended the show where Denver premiered the song, this resulted in a five-minute standing ovation.

[14] "Take Me Home, Country Roads" appeared on the LP Poems, Prayers & Promises and was released as a 45 in the spring of 1971.

[17] The song was interpolated in "Lonely Road" by Machine Gun Kelly and Jelly Roll in 2024.

[18] On November 1, 2017, the West Virginia Tourism Office announced it had obtained the rights to use "Take Me Home, Country Roads" in its marketing efforts.

"It highlights everything we love about our state: scenic beauty, majestic mountains, a timeless way of life, and most of all, the warmth of a place that feels like home whether you've lived here forever or are just coming to visit."

The song is also played after every home victory and fans are encouraged to stay in the stands and sing along with the team.

[20] On September 6, 1980, at the invitation of West Virginia Governor Jay Rockefeller, songwriters Danoff, Nivert, and Denver performed the song during pregame festivities to a sold-out crowd of Mountaineer fans.

This performance marked the dedication of the current West Virginia University Mountaineer Field and the first game for head coach Don Nehlen.

This version was first released in Germany on May 21, 2001,[39] and was issued in the United Kingdom on December 3, 2001, where it was a contender for the 2001 Christmas number-one single.

A cover version of the song, a collaboration between Copilot Music and Sound and the vocal group Spank,[72] was commissioned for and featured in both the teaser and full E3 2018 trailers for the 2018 video game Fallout 76, with its plot events set in West Virginia.

[75] In Australia, a promotional Fallout 76 vinyl featuring the cover was included with the December 2018 issue of STACK Magazine exclusively from retailer JB Hi-Fi.

[77] Jamaican reggae group Toots and the Maytals recorded a cover version for their 1975 US re-release of their 1973 album Funky Kingston.

[78] American singer-songwriter Lana Del Rey released a piano cover of the song on December 1, 2023.