It has a distribution from Esmeraldas Province in northwestern Ecuador, through western Colombia and Panama to the most southerly part of Costa Rica.

Scientists attribute this to deforestation and habitat fragmentation associated with logging, agriculture, livestock grazing, and urbanization.

[3] Cruziohyla Calcarifer secrete a chemical mucus which is said to have properties which can aid humans in fighting off bacterias.

[[Category:Amphibians of Cuesta, S. A., Reinoso, C., Morales, F., Pilaquinga, F., Morán-Marcillo, G., Proaño-Bolaños, C., Blasco-Zúñiga, A., Rivera, M., & Meneses, L. (2021).

Novel antimicrobial cruzioseptin peptides extracted from the splendid leaf frog, Cruziohyla calcarifer.