Cryostasis (clathrate hydrates)

The term cryostasis was introduced to name the reversible preservation technology for live biological objects which is based on using clathrate-forming gaseous substances under increased hydrostatic pressure and hypothermic temperatures.

Cryostasis utilizes clathrate-forming gases that penetrate and saturate the biological tissues causing clathrate hydrates formation (under specific pressure-temperature conditions) inside the cells and in the extracellular matrix.

These "cages" are unstable when empty, collapsing into conventional ice crystal structure, but they are stabilised by the inclusion of the gas molecule within them.

Most low molecular weight gases (including CH4, H2S, Ar, Kr, and Xe) will form a hydrate under some pressure-temperature conditions.

[3] Clathrates formation will prevent the biological tissues from dehydration which will cause irreversible inactivation of intracellular enzymes.