The Women's road time trial T1-2 road cycling event at the 2016 Summer Paralympics took place on the afternoon of 14 September at Flamengo Park, Pontal.

7 riders competed over one lap of a fifteen kilometre course.

[1] The T1 category is for cyclists with significant balance issues.

The T2 category is for cyclists with moderate balance issues.

Women's road time trial T1-2.