For five seasons, Darlow was an ensemble player and announcer on the children's educational series Square One Television.
On television, she has made numerous appearances in three of the different Law & Order series, and guest starred on Soul Man and The Sopranos.
In 1990, she played the part of Marge in Sesame Street Home Video Visits the Firehouse.
In 2017, Darlow began playing the recurring role of Mrs. Moskowitz on the Amazon Prime comedy-drama series The Marvelous Mrs.
Darlow has many on- and off-Broadway credits, including Grease, Prelude to a Kiss, Rumors, Taller Than a Dwarf and Trouble in Paradise.