[2] Originally located in The Northwest Mall, the organization began construction of their new and current building at 4920 San Jacinto Street in 2002.

The third floor consists of Pilsen Hall, the museum's main exhibit area, and Comenius Library.

[5] The collection includes In 2018, astronaut Andrew Feustel brought a copy of Petr Ginz's Moon Landscape drawing to the International Space Station.

On February 1, 2020, Feustel donated the copy to Czech Center Museum Houston, which is now on display on the third floor.

Inspired by Ginz's story, Ilan Ramon – whose grandfather and other family members were murdered in the Holocaust – took Moon Landscape with him on Space Shuttle Columbia.