[8] Czech canoeists qualified boats in all four classes for the Games through the 2019 ICF Canoe Slalom World Championships in La Seu d'Urgell, Spain.

Aneta Holasová booked a spot in the women's individual all-around and apparatus events, by finishing eleventh out of the twenty gymnasts eligible for qualification at the 2019 World Championships in Stuttgart, Germany.

Martin Vlach secured his selection in the men's event with a bronze-medal finish and third among those eligible for Olympic qualification at the 2019 European Championships in Bath, England.

[22] M = Medal race; EL = Eliminated – did not advance into the medal race Czech shooters achieved quota places for the following events by virtue of their best finishes at the 2018 ISSF World Championships, the 2019 ISSF World Cup series, European Championships or Games, and European Qualifying Tournament, as long as they obtained a minimum qualifying score (MQS) by 31 May 2020.

Rio 2016 Olympian Lubomír Jančařík scored a first-match final triumph to book one of the four available places in the men's singles at the 2021 ITTF World Qualification Tournament in Doha, Qatar.

[28] Meanwhile, Pavel Širuček reserved the last of the five Olympic slots available in the men's singles through a repechage play-off at the European Qualification Tournament in Odivelas, Portugal, thereby joining Jančarík on the country's roster for his maiden Games.

[29] On the women's side, Hana Matelová secured a singles spot as one of the top-ten table tennis players vying for qualification in the ITTF Olympic Rankings of June 1, 2021.

[32] Czech Republic qualified one wrestler for the men's Greco-Roman 97 kg into the Olympic competition, by progressing to the top two finals at the 2021 World Qualification Tournament in Sofia, Bulgaria.