[1] Maslany performed in the play Stupid Fucking Bird by Aaron Posner, and in particular for the Canadian premiere in 2013.

As an actor, Maslany has played parts in Robin Hood, Peter Pan, The Alice Nocturne, A Midsummer Night's Dream, George Dandin, and Pride and Prejudice at the Globe Theatre in Regina; Rage, The Alice Nocturne, and The Story of Mr. Wright in the Globe Theatre Shumiatcher Sandbox Series; as well as Wrecked, and The Secret Life of the Octopus, as part of the Persephone Youth Tour.

Maslany made his on-screen debut in the 2000 film Skipped Parts, playing character Petey Pierce.

[6] Joining the cast of the series Murdoch Mysteries in 2017, Maslany took on the character of Llewellyn Watts, a police detective.

[2][8] Daniel Maslany worked as executive producer, second assistant director and composer for the short film, Shut Up (2019).