The Darling Mills Creek, an urban watercourse[1] that is part of the Parramatta River catchment, is located in Greater Western Sydney, New South Wales, Australia.

[2] The course of the creek is approximately 10 kilometres (6.2 mi); and the creek passes through the Cumberland State Forest, the Darling Mills State Forest, Bidjigal Reserve, Excelsior Park, and many smaller reserves and parks.

[1] The Darling Mills Creek is transversed by the Cumberland Highway, the M2 Hills Motorway,[3] and marks the boundary between Windsor Road and Church Street in North Parramatta.

In 1825, the Darling Flour Mills were built for John Raine, upstream on the Windsor Road.

Part of the facade of the building remains on the site, which was occupied by Bunnings's North Parramatta store in the early 21st century.