Dean Brook is a river in Smithills, Bolton, Greater Manchester, historically within Lancashire.

Dean Brook rises on Smithills Moor and flows in a south-easterly direction, through a deep valley called High Shores Clough.

The Dean Brook in Smithills, Bolton, should not be confused with a different Dean Brook, which rises on the north-facing slopes of Rivington Moor, flows in a westerly direction just north of Rivington, Lancashire, and empties into Upper Rivington Reservoir.

It should also not be confused with Deane Church Brook, also known as the Kirk Brook, which flows through Deane Church Clough in the west of Bolton.

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