[2] The show is narrated by fictional hosts named Wiz and Boomstick, respectively voiced by series creators Ben Singer and Chad James.

[4] Wiz is a genius scientist who presents scientific information and mathematical calculations, while Boomstick is a street-smart redneck that discusses weapons, armor, and feats.

[2][5] Each episode of Death Battle features a matchup between fictional characters from different media that have similar powers, skills, or backstories.

The majority of each episode showcases background information about each character while analyzing their abilities and feats, and ends with a fully-animated battle scene that decides the winner of the matchup, taking into account the prior analysis.

ScrewAttack created the series Desk of Death Battle, which showcases pop-culture trivia that the ScrewAttack team found interesting while researching and writing the main show,[12] presented by Death Battle supporting character Jocelyn the Intern, who is voiced by Lisa Foiles.

[6][25] Following the closure of Rooster Teeth, fans of Death Battle speculated about the show's future, and the hashtag "#SaveDeathBattle" trended on Twitter.

Death Battle is one of the most popular web series dedicated to battleboarding, and the show's YouTube channel has been described as a "central hub" for fans of the genre.