In the analysis of the molecular formula of organic molecules, the degree of unsaturation (DU) (also known as the index of hydrogen deficiency (IHD), double bond equivalents (DBE), or unsaturation index[1]) is a calculation that determines the total number of rings and π bonds.
The final structure is verified with use of NMR, mass spectrometry and IR spectroscopy, as well as qualitative inspection.
The formula for degree of unsaturation is: where ni is the number of atoms with valence vi.
In either case, oxygen and other divalent atoms do not contribute to the degree of unsaturation, as 2 − 2 = 0.
A popular form of the formula is as follows: where C, N, H and X represent the number of carbon, nitrogen, hydrogen and halogen atoms, respectively.