He played college football for the Washington Huskies and was selected by the Atlanta Falcons in the first round of the 2013 NFL draft.

[9] Head coach Mike Smith named Samuel and Trufant the starting cornerbacks to begin the regular season.

[10] He made his professional regular season debut and first career start in the Atlanta Falcons' season-opener against the New Orleans Saints.

In Week 7, Trufant recorded a season-high seven combined tackles and broke up a pass during a 31–23 victory against the Tampa Bay Buccaneers.

[17] On November 9, 2014, Trufant recorded a season-high seven combined tackles and a pass deflection during their 27–17 victory against the Tampa Bay Buccaneers.

[19] Trufant finished the season with 61 combined tackles (53 solo), 16 pass deflections, and three interceptions, playing in 16 games with 16 starts.

[20] The Falcons' new head coach, Dan Quinn, retained Trufant and Alford as the starting cornerback duo.

[21] In Week 11, Trufant recorded four combined tackles, two pass deflections, and his first career sack on quarterback Matt Hasselbeck during a 21–24 loss to the Indianapolis Colts.

[22] He finished the season with 42 combined tackles (35 solo), 11 pass deflections, a sack, and an interception, playing in 16 games with 16 starts.

On October 16, Trufant made five combined tackles and a sack on Russell Wilson in the Falcons' 26-24 loss at the Seattle Seahawks.

[24][25] On November 29, Atlanta placed Trufant on injured reserve after they discovered he would need shoulder surgery, causing him to miss his first game in four seasons.

[7][30][31] New defensive coordinator Marquand Manuel, who replaced Rick Smith after the Falcons' collapse in Super Bowl LI.

In Week 2, Trufant recorded two solo tackles, broke up a pass, an interception, and recovered a fumble off a strip sack by Vic Beasley and returned it 15-yards for a touchdown during a 34–23 victory against the Green Bay Packers on NBC Sunday Night Football.

[34] He finished the 2017 season with 41 combined tackles (36 solo), 12 pass deflections, two interceptions, a sack, a fumble recovery, and a touchdown in 15 games and 15 starts.

[36] Trufant missed four games in the middle of the season due to a toe injury he suffered during Week 5 against the Houston Texans.