4 mg/kg (mouse, intravenous)[3] Digitonin is a steroidal saponin (saraponin) obtained from the foxglove plant Digitalis purpurea.

Its aglycone is digitogenin, a spirostan steroid.

It has been investigated as a detergent, as it effectively water-solubilizes lipids.

As such, it has several potential membrane-related applications in biochemistry, including solubilizing membrane proteins, precipitating cholesterol, and permeabilizing cell membranes.

[4][5] Digitonin is sometimes confused with the cardiac drugs digoxin and digitoxin; all three can be extracted from the same source.