It is now a suburb of Kitchener, Ontario, home to the Doon Heritage Village and the main campus of Conestoga College.

Doon was established in a forested area around Schneider Creek where it enters the Grand River from the south.

Richard Beasley sold 3,600 acres (1,500 ha) of this land to John Biehn Sr. of Montgomery County, Pennsylvania, in 1800.

[2] In the 1830s the Ferrie family moved to the area, where they established several businesses in what is now Lower Doon including a distillery, tavern, general store, saw mill, cooperage, blacksmith shop and kiln.

Doon Mills, driven by water, was fully operational in 1839, grinding oatmeal, flour, and barley for the local population.

In Tow Town, Moses and Joseph Perine established a sawmill and a flax mill that made rope and twine, the first in Canada of this nature.

[2] The Doon Twine and Cordage factory opened in 1856 making products from locally-grown flax and hemp.

By the early 1890s there was a post office, grocer and baker, two tailors, a cooper, blacksmith, shoemaker, wagon maker, bridge builder and scissors manufacturer.

In 1854 the Doon Presbyterian Church was opened on land donated by Robert Ferrie and built with his financial assistance.

The Grand Trunk Railway's east–west mainline was constructed to the north of Doon through Berlin,[6] which by then had become the county seat.

The Great Western Railway, a major competitor of the Grand Trunk, built a set of branch lines managed by a complex set of subsidiary companies northward from a point on its own mainline at Harrisburg, in what has been described as both Canada's first railway junction and its first branch line.

[12] In the 1930s, amidst the Great Depression, passenger service ended on the line and the Doon and Blair stations were closed.

Regional Road 28 passes through Doon, where it is known as Homer Watson Boulevard until becoming Fountain Street South within Cambridge.

Conestoga College's Doon Campus is a minor hub for Grand River Transit bus service.

With internal traffic congestion on the campus increasing, a dedicated on-campus bus station has been proposed, which would be directly accessible from Doon Valley Drive, a public roadway.

[20] Homer Watson (1855–1936) was a self-taught painter who devoted most of his life to painting landscapes of the country of Doon in a combination of romantic and realistic styles.

[23] She became curator of the Homer Watson Art Gallery in Doon, and held this post until her death on 22 October 1947.

To accommodate growth and reduce transportation costs, in 1916 the operation was moved from Doon to a larger factory in Kitchener.

[26] Hilda Ranscombe (1913–1998) was born in Doon, and became a star ice hockey player on the Preston Rivulettes.

[32] Hugh C. Elliott (1899-1995), who had served in 1918–19 with the Canadian Engineers in Vladivostok, Siberia, was a charter director of the Doon Pioneer Village.

[34] The original campus of Conestoga College, and the largest, is located in Doon by the Highway 401 / Homer Watson Blvd.