Almost from its foundation, the monastery held stauropegic status,[4] that is, directly attached to the Patriarch of Constantinople.

During the Ottoman period, the monastery owned numerous lands located on Mount Penteli, as well as in other regions of Attica.

The monastery was destroyed in the aftermath of the Venetian occupation of Athens in 1688–1690, and then during the Greek War of Independence.

From the outside, its high walls and heavy doors give it the appearance of a fortress.

The monastery celebrates the 15th (Dormition of the Theotokos) and 16 August (memory of the founder, Saint Timotheos), as well as the Monday of the feast of the Holy Spirit.