The 107,000 square feet (9,900 m2) main building sits on a 17-acre (6.9 ha) site adjacent to the South Platte River.

Its freshwater and marine aquaria total approximately 1,000,000 US gallons (3,785,000 L), and exhibit a variety of fish and other animals.

The facility opened June 21, 1999 and soon earned accreditation by the Association of Zoos and Aquariums (AZA).

The Indonesia River Journey included exhibits of animals such as Asian arowanas, rainbowfish, and endangered Sumatran tigers.

A volunteer program administered by the Deep Blue Sea Foundation, a nonprofit group, is in place.

The Deep Blue Sea Foundation was formed after Landry's purchased the aquarium to ensure the educational goals of the original founders would continue to be met.