It was read as an audiobook by British comedian Rik Mayall as part of the 2000 audio release The Dr. Seuss Collection.

[1] Peter Lewis, writing for Common Sense Media, gave the book positive reviews, stating "Dr. Seuss turns the alphabet from fifty-two shapes and twenty-six sounds one has to memorize into an exercise in rhymery and wordplay.

Uppercase and lowercase letters are taken for a spirited airing, matched with an apt selection of fun words, all set in the suitably absurd world of Seuss characters and creatures".

The letters each take a turn in the spotlight and then are wedded to a few well-chosen words that convey the Seuss worldview of the high humor to be found in mental play: 'Many mumbling mice are making midnight music in the moonlight ... mighty nice.'")

McCrillis Collection by Alesandra M. Schmidt, she references "Ten tired turtles on a tuttle-tuttle tree" in Case 8, stating, while comparing the book to Apricot ABC by Miska Miles, "Ecolological awareness of the 1960s is evident in the imaginative story of an apricot that falls to the ground in a meadow (#48).

"[7][8] In the 1995 CD-ROM game by Living Books, Ichabod and Izzy appear on every page and they find out more things that also begin with each letter.

[10][11] In Journal of the American Society of Echocardiography, James N. Kirkpatrick, MD, director of the Echocardiography Laboratory at the UW Medical Center and associate professor of medicine, uses the letters E for Echocardiography, S for Sinister structural shortcomings, and C for Congestive heart failure from the book in ABCD's of Heart Failure: Echo-ing through the First Stage to outline the stages of heart failure, stating, "In his insightful and witty educational offering, Dr. Seuss’s ABC, Theodor Seuss Geisel 1 poses the intriguing rhetorical question “Big A, little a, what begins with A?” He might have answered, “An amalgamated assemblage of advancing ailments affecting the heart” (it works better if you consider the h to be silent.)