Duboistown (pronounced 'doo-BOYS town') is named for its founders John and Mathias DuBois who bought 489 acres (1.98 km2) of land between 1852 and 1857.

The DuBois brothers divided their land into parcels and established the village that bears their name.

John DuBois left the West Branch Susquehanna Valley before Duboistown was established as a borough.

The town built by the DuBois brothers and established as a borough in 1878 is by no means the beginning of the history of Duboistown.

A tribe of Susquehannock Indians had what appears to have been a fairly major settlement at the mouth of the creek.

He purchased several tracts of land beginning in 1773, including the parcel owned by Samuel Boone, near the mouth of Mosquito Run.

After the Battle of Wyoming in the summer of 1778 (near what is now Wilkes-Barre) and smaller local attacks, the "Big Runaway" occurred throughout the West Branch Susquehanna valley.

Settlers abandoned their homes and fields, drove their livestock south, and towed their possessions on rafts on the river to Sunbury.

He quickly built a tavern in which the weary farmers could enjoy a drink and get some food while they waited for their grain to be ground into flour.

Today Culbertson's Mill and tavern are long gone and the area is a largely overgrown riverbank with an abandoned softball field, that is surrounded by a railroad, bridge, and woods.

The 200 block of Summer Street in Duboistown is decorated for Christmas each December and is known as Candy Cane Lane.