[1] Inhibition causes an overly high accumulation of acetylcholine between the nerve and muscle cells.

[2] Patent assigned to US army for EA-3990 among other similar nerve agents was filed in December 7, 1967.

In general their penetration through the blood-brain barrier is difficult due to quaternary nitrogens in these molecules.

[1] For VX, the median lethal dose (LD50) for 70 kg men via exposure to the skin is estimated to be 10 mg, and the lethal concentration time (LCt50), measuring the concentration of the vapor per length of time exposed, is estimated to be 30–50 mg·min/m3.

[3] EA-3990's CAS is 110913-95-6, mass 718.7 g/mol,[1] melting point 190–191 °C,[3] density 1.33 g/cm3, vapor pressure is negligible, and it is soluble in alcohols, acetic acid and chloroform.

Other secondary amines can be used, such as those containing methyl, ethyl, propyl, isopropyl, butyl and benzyl groups.

1 mol α,ω-dihaloalkane (e.g. 1,8-dibromooctane in this case) in acetonitrile is heated on a steam bath for 6 hours.