The enzyme-linked immunosorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single cell.
The FluoroSpot Assay uses fluorescence in order to analyze multiple analytes, meaning it can detect the secretion of more than one type of protein.
In 1988, Czerkinsky developed an ELISA spot assay that quantified the secretion of a lymphokine by T cells.
In the same year, dual-color ELISpot was combined with computer imaging for the first time, which allowed for the enumeration and analysis of spots.
You can also analyze the IFN-y cytokine and granzyme B secretion in order to assess cytotoxic T-cell responses.