Matos was born in San Juan, Puerto Rico and graduated from North Hunterdon High School in New Jersey in June 1996.
After a few guest appearances in various prime-time shows, Matos originated the role of Ricky Garza on ABC's daytime soap opera Port Charles.
After the show was canceled in 2003, Matos continued to do guest appearances, before landing the role of Professor Pete Marquez on ABC's General Hospital.
Matos went on to star in the CBS series Cane, alongside Jimmy Smits, Hector Elizondo, and Rita Moreno.
Eddie Matos joined the cast of All My Children in 2010, playing the role of Reverend Ricky Torres.