He painted historical events, Biblical scenes, and portraits, and was in great demand for decorating public celebrations.
The novelist, Hendrik Conscience, was a good friend of his and he provided illustrations for several of his works, including The Lion of Flanders.
In 1846 Dujardin traveled to Italy with Eugène Van Havre and made an extensive report about his whereabouts.
Unlike many of his colleagues at the academy, he supported Flemish movement and was a board member of "Voor Tael en Kunst", an organization that promoted cultural and entertainment activities.
In 1855, he was one of the co-founders of the magazine De Vlaemsche School, which served as a successor to "Voor Tael en Kunst".