When the Catholic Mary I of England came to the throne, his father's position became untenable, and he retired to Ipswich close to the family estates at Freston.
In the next year, Elizabeth I of England came to the throne and William Latymer was restored to his former positions, and became the Treasurer of Westminster Abbey.
He went to study at St John's College, Cambridge at the age of fourteen and left in 1575 (there is no record of his graduation).
He lived most of his adult life at 'the Signe of the Cocke' in Ramme Alley in the Parish of St Dunstan's in the City of London.
His relations challenged his will in the Court of Chancery, resulting in a seven-year delay before the bequests could take effect while the case was fought.