This species was first described in 2023 and named in honor of German naturalist Alexander von Humboldt.

[1] Eleutherodactylus humboldti has a triangular head and measures 23.4–25.7 mm (0.92–1.01 in) in snout-vent length.

[1][2] Its dorsal surfaces have small tubercles and are colored a brownish tan, whereas its ventral surfaces are translucent brownish tan with white tipped small tubercles on the belly and thighs.

[1] This species is found in clearings in pine-oak woodland in the Mil Cumbres region of the Eje Neovolcánico range.

The three type specimens were found at an elevation of 1,982 m (6,503 ft) above sea level at night, calling from low vegetation.