Eliezer Zweifel (1815–1888) (Hebrew: אליעזר צבי צְוַויפֶל) was a Russian-Jewish writer who was associated with the Jewish Enlightenment movement (haskalah).

While in his twenties, Zweifel moved to Odessa, where he worked as a religious preacher and tutor for sixteen years.

From 1853 to 1873, Zweifel was appointed as a Talmud teacher at Beth Midrash L'Rabbanim in Zhytomyr, an institution associated with the Jewish Enlightenment movement (haskalah).

Zweifel's writings often contained anti-Haskalah statements and drew criticism from other Haskalah authors including Mendele Mokher Sefarim.

Zweifel authored a number of books and contributed dozens of essays to the Jewish periodicals of his time.