She switched her focus to basketball before her freshman year at Northwest Guilford High School in Greensboro, North Carolina, when she stood 6 ft 2 in (1.88 m).

[15] Kitley sustained a right shoulder injury against North Carolina in the quarterfinals of the 2022 ACC tournament and was sidelined as her team lost to NC State in the following game.

[18] On February 23, 2023, Kitley set the Virginia Tech career scoring record, while posting 21 points and 10 rebounds and making the game-winning shot at the buzzer, in a 61–59 win over North Carolina.

Kitley repeated as ACC Player of the Year, made the WBCA All-America team and was named a second-team All-American by the AP and USBWA.

[23] After the spring 2023 semester, Kitley completed her Bachelor of Science in human nutrition with concentrations in foods and exercise and summa cum laude honors.

[25] On March 3, 2024, in the regular season finale against the University of Virginia, Kitley suffered an ACL injury in her left leg that would sideline her indefinitely, ending her collegiate basketball career.

Elizabeth Kitley finished her career at Virginia Tech with all-time program records in games started, minutes played, points scored, field-goal percentage, field goals made, double-doubles, rebounds, and blocks.

The ceremony will take place in the 2024-25 NCAA Division I Women's College Basketball season during a game when the Hokies play at Cassell Coliseum, their home venue.

The Las Vegas Aces, upon drafting Kitley, reserved her contract rights as one of two (2) maximum inactive players (ACL) through the duration of the 2024 WNBA season.