It is hosts the primary diplomatic mission from Ukraine to the Italian Republic.

[3] Italy also supported Ukraine's sovereignty over the Crimean Peninsula in the 2014 annexation of Crimea by the Russian Federation.

The DPR and LPR declared independence from Ukraine in 2014 with the help of Russia, however Ukraine considers them to be rebellious provinces whose declarations of independence lacked legal basis (see War in Donbass and Russo-Ukrainian War).

On a visit to Italy in February 2020, Ukrainian President Volodymyr Zelenskyy urged Italian Prime Minister Giuseppe Conte to shut down Italy's "illegal" diplomatic offices in the DPR and the LPR.

[5] In addition to the Ukrainian embassy in Rome, Ukraine maintains consulates in the Italian cities of Florence, Bari, and Milan.