The embassy's chancery is situated in the Palazzo Margherita, Via Vittorio Veneto, Rome.

The United States also maintains consulates general in Milan, Florence and Naples, and consular agencies in Genoa, Palermo, and Venice.

[3] The current United States Ambassador to Italy is former Delaware Governor Jack Markell.

The Embassy of the United States to the Holy See, previously located on Aventine Hill, moved to new headquarters in September 2015 in a separate building on the same compound as the United States Embassy Rome,[5] while the United States Mission to the UN Agencies in Rome is located in a third building on the same compound since December 2011, when they moved from their former location at Piazza del Popolo.

Junzo Okudaira, a member of the Japanese Red Army, was suspected to have carried out the attack.