[2] Embryo rescue is most often used to create interspecific and intergeneric crosses that would normally produce seeds which are aborted.
Many times, remote hybridizations will fail to undergo normal sexual reproduction, thus embryo rescue can assist in circumventing this problem.
[9] Embryos are manually excised and placed immediately onto a culture medium that provides the proper nutrients to support survival and growth (Miyajima 2006).
While the disinfestation and explant excision processes differ for these three techniques, many of the factors that contribute to the successful recovery of viable plants are similar.
The main factors that influence success are; the time of culture, the composition of the medium, and temperature and light.