Murashige and Skoog medium

Along with its modifications, it is the most commonly used medium in plant tissue culture experiments in the laboratory.

[1] As Skoog's doctoral student, Murashige originally set out to find an as-yet undiscovered growth hormone present in tobacco juice.

A series of experiments demonstrated that varying the levels of these nutrients enhanced growth substantially over existing formulations.

It was determined that nitrogen in particular enhanced growth of tobacco in tissue culture.

According to recent scientific findings, however, MS medium is not suitable as a nutrient solution for deep water culture or hydroponics.

Mammillaria sp. on MS media in agar
Mammillaria miegiana on liquid MS media