PROTEIN-x1-x2-x3-x4-x5-x6-x7-x8-x9-x10-x11-...-xn The mechanism is an enzymatic hydrolysis that requires two critical molecules; a proton donor (most likely an acid) and a nucleophile(most likely a base).
[2] The Endoglycosidases mechanism has two forms; an acid catalyzed protonation of the glycosidic oxygen yielding stereochemical retention at the anomeric carbon or an acid catalyzed protonation of the glycosidic oxygen with a concomitant attack of a water molecule activated by the base residue yielding a stereochemical inversion.
This new mutated enzyme when exposed to the proper compounds will undergo oligosaccharide synthesis and will not hydrolyze the newly formed polymer chains.
[5] Endoglycosidases also have potential application in fighting autoimmune diseases such as arthritis and systemic lupus erythematosus.
In 2008, a team of researchers demonstrated that injection of endgoglycosidase S “efficiently removes the IgG-associated sugar domain in vivo and interferes with autoantibody-mediated proinflammatory processes in a variety of autoimmune models.”[6] Clearly the manipulation and mutation of this enzyme holds great promise for being able to fight a variety of diseases in the body.