Endospore staining

Endospores contain a tough outer coating made up of keratin which protects them from nucleic DNA as well as other adaptations.

He found a differential staining technique where endospores appear green and vegetative cells appear pinkish red.

Schaeffer and Fulton made the heating process a lot faster by using a Bunsen burner.

This improved method provided a quicker and easier test and allowed for the spores to be more susceptible to the dyes.

[7] In the end, a proper smear would show the endospore as a green dot within either a red or pink-colored cell.

This is due to its waxy cell wall which retains the malachite green dye even after the decolorizing process.