[1] The ship's fittings were carved by Sebastian Vicars, and painted and gilded by Robert Peake and Paul Isackson between Easter and Michaelmas 1611.

By 1660 she was carrying 80 guns, and with the English Restoration of King Charles II she resumed the name Royal Prince.

In 1663 she was rebuilt again at Woolwich Dockyard by Sir Phineas Pett II as a 92-gun first-rate ship of the line.

[3] In 1665, during the Second Anglo-Dutch War, she served as the flagship of Edward Montagu, 1st Earl of Sandwich at the Battle of Lowestoft on 3 June.

When Dutch fireships surrounded the stranded ship, the crew panicked and Ayscue was forced to surrender to Lieutenant-Admiral Cornelis Tromp who was aboard the Gouda.