[1] At the age of 11, Ambrose moved from AA to AAA boys hockey, and in her second season, she was named team captain.

[4] In addition, she participated in the Canada Celebrates Event on June 30 in Edmonton, Alberta which recognized the Canadian Olympic and World hockey champions from the 2009–10 season .

[9][10][11] On January 16, 2012, it was announced that Ambrose committed to join the Clarkson Golden Knights women's ice hockey program in autumn 2012.

[13] To begin her sophomore season, Ambrose amassed five and four-point performances, including hat tricks in consecutive games.

Becoming the ninth player in program history to reach the century club, she achieved the feat on January 10, 2015, scoring a goal in a 1–1 draw versus conference rival Cornell.

In the previous game (a February 27 tilt with the Cornell Big Red), Ambrose recorded four assists, her best offensive output for the season.

After being released from Hockey Canada's Centralization camp, Ambrose was traded from the Toronto Furies to Les Canadiennes de Montreal.

[20] In October 2020, Ambrose wrote a piece for Hockey Canada detailing her struggles with depression and anxiety, also revealing her same-sex preference.