[2] The route travels east into Denton County and into the city of Justin; FM 407 was the primary access route between the former US 77 and farms that were part of the original Icarian Colony, where the city of Justin now sits.

Near Bartonville at Country Club Road, FM 407 (locally known from this point as Justin Road) previously turned south before resuming an eastward path; however, as of 2014 407 was re-routed to the north, eliminating the required turns in either direction and the ensuing bottleneck during rush hour congestion (similar to a re-route in 2012 near Lantana, as part of a widening to four lanes, which by-passed a four-way stop).

It runs near the city limits of Double Oak, Copper Canyon, Flower Mound, and Highland Village, and is a major artery for new developments between the two forks of Interstate 35 just south of Denton.

[4] FM 407 then enters northern Lewisville, where state maintenance ends at an interchange with I-35E.

[1] The TxDOT designation of the section of FM 407 between FM 1830 in Bartonville to I-35E was officially changed to Urban Road 407 (UR 407) on June 7, 1995; nonetheless, as with other urban roads, TxDOT continued to sign the route using the traditional Farm Road marker.