Fernando Afonso of Portugal (1135 – 1 March 1207) was the twelfth Grand Master of the Knights Hospitaller, serving between 1202–1206.

[3] Fernando Afonso was for a short period of time alferes-mor of the Kingdom of Portugal.

He became Master of the Knights Hospitaller in the Iberian Peninsula in 1198, as part of the Langue de Castille, León and Portugal.

The only highlight of his magisterium was the promulgation of a new statute drawn up during the General Chapter, for which he was reproached.

His remains were buried in Santarém, at the Church of São João de Alporão.