Deriving from the Grand Chapelle of Philip I of Castile, the choir served under various forms until the reign of Charles II of Spain.
After Margaret died Charles appointed his sister governor of the Netherlands 1531–1555 at Brussels, where Benedictus Appenzeller was master of the court chapel.
Charles V, Holy Roman Emperor attained his majority in 1515, allowing him to rule the Burgundian territories he had inherited.
He gained the throne of Spain in 1517 along with the chapel choir of his grandmother Isabel the Catholic, and then in 1519 Charles became Holy Roman Emperor.
His maestros de capilla included Adrien Pickart, Thomas Crecquillon, Cornelius Canis and Nicolas Payen.
Aside from Burgundian-Flemish repertory, the chapel performed music of the Roman (e.g. Palestrina) and Venetian (e.g. Andrea Gabrieli) schools.
[1] Philip IV of Spain had been taught as a child by Romero, who was succeeded as maestro in 1634 by Carlos Patiño, who was promoted from the junior capilla real at the Royal Convent of La Encarnación.
Though the chapel took second place to the italianate court and theatre music of Joseph de Torres, Antonio Literes, and Sebastián Durón.