Directed by Jerry Lopez Sineneng and Rechie del Carmen, it stars Eliza Pineda in the title role.

The series is based on the 1970s radio drama Flordeluna, written by Marcos Navarro Sacol and its television adaptation by RPN in 1978, starring Janice de Belen in the title role.

The series theme song is "Maria Flordeluna" and was written by Arden Condez and Jonathan Manalo.

Maria Flordeluna was the only primetime drama series in 2007 on either ABS-CBN or GMA to have gained viewers through the duration of its whole run, registering 42.3% based on average monthly nationwide ratings).

[2] The show's finale was notable during the same period that GMA's viewership lead in Mega Manila was pitted against ABS-CBN's dominant audience shares in both Visayas and Mindanao in the combined nationwide ratings.