She succeeded her father in 1362 as a young widow with only one son, and attracted many suitors, for which reason W. Miller labelled her "the Penelope of Frankish Greece".
This marriage was vetoed by the Republic of Venice, who regarded it of the utmost importance that she married a Venetian so as to prevent any potential anti-Venetian establishment in the Duchy.
Venice protested, but the groom was supported by the Kingdom of Naples through Robert of Taranto, who, as Prince of Achaia, was the suzerain of Athens.
There, she was treated with respect due to her rank but informed that she would never be allowed to leave unless she agreed to marry the Venetian candidate, her cousin Nicholas Spezzabanda.
Florence died in 1371 and was succeeded by her son by Giovanni, Nicholas III dalle Carceri, under the regency of his stepfather during his minority.