Both segments lie entirely within 4 miles (6 km) of Florida's northern boundary.

The western segment of SR 2 extends 63 miles (101 km) from its western terminus (an intersection with SR 81) south of Sweet Gum Head, Florida to the Georgia border near Bascom, Florida.

The predominantly rural SR 2 passes through the towns of Pittman, Noma, Campbellton, and Malone as it crosses Holmes and Jackson Counties in the Florida panhandle.

With most of the roadway constructed adjacent to a track maintained and operated by the Norfolk Southern Railway, it serves no Florida municipalities (although Taylor, Florida is ten miles (16 km) to the south in Baker County).

SR 2 clips the northeastern corner of Columbia County with the segment's westernmost mile.